Proteomic analysis of Moroccan cobra Naja haje legionis venom using tandem mass spectrometry.
Identifieur interne : 000216 ( Main/Exploration ); précédent : 000215; suivant : 000217Proteomic analysis of Moroccan cobra Naja haje legionis venom using tandem mass spectrometry.
Auteurs : Ibtissam Malih [Maroc] ; Muhamad Rusdi Ahmad Rusmili [Malaisie] ; Ting Yee Tee [Malaisie] ; Rachid Saile [Maroc] ; Noreddine Ghalim [Maroc] ; Iekhsan Othman [Malaisie]Source :
- Journal of proteomics [ 1876-7737 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Elapidae, Venins des élapidés.
- Animaux, Protéomique, Spectrométrie de masse en tandem, Spécificité d'espèce.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Elapid Venoms.
- metabolism : Elapidae.
- Animals, Proteomics, Species Specificity, Tandem Mass Spectrometry.
Abstract
UNLABELLED
The proteome of the venom of Naja haje legionis, the only medically important elapid species in Morocco, has been elucidated by using a combination of proteomic techniques that includes size exclusion chromatography, reverse-phase HPLC, Tricine/SDS-Page, tryptic digestion, Q-TOF tandem mass spectrometry and database search. The sequence analysis of venom fractions revealed a highly complex venom proteome which counts a total of 76 proteins identified from database that can be assigned into 9 proteins families. We report the identification of: cobra venom factor (CVF), l-amino-acid oxidases (LAAO), acetylcholinesterase (AChE), snake venom metalloproteinases (SVMP), cysteine rich secretory proteins (CRISP), venom nerve growth factor (vNGF), phospholipases A2 (PLA2), vespryns, kunitz-type inhibitor, short neurotoxins, long neurotoxins, weak neurotoxins, neurotoxin like proteins, muscarinic toxins, cardiotoxins and cytotoxins. Comparison of these proteins showed high sequence homology with proteins from other African and Asian cobras. Further works are needed to assess the contribution of individual toxins in venom toxicity.
BIOLOGICAL SIGNIFICANCE
Naja haje legionis is one of the medically important snakes implicated in the pathogenesis of snake bite in Morocco. The absence of information about venom composition and clinical manifestations of envenomation by this cobra represents an obstacle for the management of this environmental disease in the country. The elucidation of Moroccan cobra venom composition will provide a reasonable guidance for clinician to understand the pathophysiological conditions associated with cobra envenomation and the elaboration of better management strategies.
DOI: 10.1016/j.jprot.2013.11.012
PubMed: 24269350
Affiliations:
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Department of Biomedical Sciences, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus, Malaysia; Laboratory of Biology and Health, URAC 34, Hassan II University Mohammedia - Casablanca, Faculty of Science Ben M'sik, Morocco. Electronic address: ibtissam.malih@gmail.com.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Venom and Toxins Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco; Department of Biomedical Sciences, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus, Malaysia; Laboratory of Biology and Health, URAC 34, Hassan II University Mohammedia - Casablanca, Faculty of Science Ben M'sik</wicri:regionArea><wicri:noRegion>Faculty of Science Ben M'sik</wicri:noRegion></affiliation></author><author><name sortKey="Ahmad Rusmili, Muhamad Rusdi" sort="Ahmad Rusmili, Muhamad Rusdi" uniqKey="Ahmad Rusmili M" first="Muhamad Rusdi" last="Ahmad Rusmili">Muhamad Rusdi Ahmad Rusmili</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biomedical Sciences, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Department of Biomedical Sciences, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus</wicri:regionArea><wicri:noRegion>Monash University Sunway Campus</wicri:noRegion></affiliation></author><author><name sortKey="Tee, Ting Yee" sort="Tee, Ting Yee" uniqKey="Tee T" first="Ting Yee" last="Tee">Ting Yee Tee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biomedical Sciences, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Department of Biomedical Sciences, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus</wicri:regionArea><wicri:noRegion>Monash University Sunway Campus</wicri:noRegion></affiliation></author><author><name sortKey="Saile, Rachid" sort="Saile, Rachid" uniqKey="Saile R" first="Rachid" last="Saile">Rachid Saile</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biology and Health, URAC 34, Hassan II University Mohammedia - Casablanca, Faculty of Science Ben M'sik, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Laboratory of Biology and Health, URAC 34, Hassan II University Mohammedia - Casablanca, Faculty of Science Ben M'sik</wicri:regionArea><wicri:noRegion>Faculty of Science Ben M'sik</wicri:noRegion></affiliation></author><author><name sortKey="Ghalim, Noreddine" sort="Ghalim, Noreddine" uniqKey="Ghalim N" first="Noreddine" last="Ghalim">Noreddine Ghalim</name><affiliation wicri:level="1"><nlm:affiliation>Venom and Toxins Laboratory, Pasteur Institute of Morocco, Casablanca, Morocco.</nlm:affiliation><country xml:lang="fr">Maroc</country><wicri:regionArea>Venom and Toxins Laboratory, Pasteur Institute of Morocco, Casablanca</wicri:regionArea><wicri:noRegion>Casablanca</wicri:noRegion></affiliation></author><author><name sortKey="Othman, Iekhsan" sort="Othman, Iekhsan" uniqKey="Othman I" first="Iekhsan" last="Othman">Iekhsan Othman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biomedical Sciences, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>Department of Biomedical Sciences, Jeffrey Cheah School of Medicine and Health Sciences, Monash University Sunway Campus</wicri:regionArea><wicri:noRegion>Monash University Sunway Campus</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of proteomics</title><idno type="eISSN">1876-7737</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Elapid Venoms (metabolism)</term><term>Elapidae (metabolism)</term><term>Proteomics (MeSH)</term><term>Species Specificity (MeSH)</term><term>Tandem Mass Spectrometry (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Elapidae (métabolisme)</term><term>Protéomique (MeSH)</term><term>Spectrométrie de masse en tandem (MeSH)</term><term>Spécificité d'espèce (MeSH)</term><term>Venins des élapidés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Elapid Venoms</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Elapidae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Elapidae</term><term>Venins des élapidés</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Proteomics</term><term>Species Specificity</term><term>Tandem Mass Spectrometry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Protéomique</term><term>Spectrométrie de masse en tandem</term><term>Spécificité d'espèce</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>UNLABELLED</b></p><p>The proteome of the venom of Naja haje legionis, the only medically important elapid species in Morocco, has been elucidated by using a combination of proteomic techniques that includes size exclusion chromatography, reverse-phase HPLC, Tricine/SDS-Page, tryptic digestion, Q-TOF tandem mass spectrometry and database search. The sequence analysis of venom fractions revealed a highly complex venom proteome which counts a total of 76 proteins identified from database that can be assigned into 9 proteins families. We report the identification of: cobra venom factor (CVF), l-amino-acid oxidases (LAAO), acetylcholinesterase (AChE), snake venom metalloproteinases (SVMP), cysteine rich secretory proteins (CRISP), venom nerve growth factor (vNGF), phospholipases A2 (PLA2), vespryns, kunitz-type inhibitor, short neurotoxins, long neurotoxins, weak neurotoxins, neurotoxin like proteins, muscarinic toxins, cardiotoxins and cytotoxins. Comparison of these proteins showed high sequence homology with proteins from other African and Asian cobras. Further works are needed to assess the contribution of individual toxins in venom toxicity.</p></div><div type="abstract" xml:lang="en"><p><b>BIOLOGICAL SIGNIFICANCE</b></p><p>Naja haje legionis is one of the medically important snakes implicated in the pathogenesis of snake bite in Morocco. The absence of information about venom composition and clinical manifestations of envenomation by this cobra represents an obstacle for the management of this environmental disease in the country. The elucidation of Moroccan cobra venom composition will provide a reasonable guidance for clinician to understand the pathophysiological conditions associated with cobra envenomation and the elaboration of better management strategies.</p></div></front></TEI><affiliations><list><country><li>Malaisie</li><li>Maroc</li></country></list><tree><country name="Maroc"><noRegion><name sortKey="Malih, Ibtissam" sort="Malih, Ibtissam" uniqKey="Malih I" first="Ibtissam" last="Malih">Ibtissam Malih</name></noRegion><name sortKey="Ghalim, Noreddine" sort="Ghalim, Noreddine" uniqKey="Ghalim N" first="Noreddine" last="Ghalim">Noreddine Ghalim</name><name sortKey="Saile, Rachid" sort="Saile, Rachid" uniqKey="Saile R" first="Rachid" last="Saile">Rachid Saile</name></country><country name="Malaisie"><noRegion><name sortKey="Ahmad Rusmili, Muhamad Rusdi" sort="Ahmad Rusmili, Muhamad Rusdi" uniqKey="Ahmad Rusmili M" first="Muhamad Rusdi" last="Ahmad Rusmili">Muhamad Rusdi Ahmad Rusmili</name></noRegion><name sortKey="Othman, Iekhsan" sort="Othman, Iekhsan" uniqKey="Othman I" first="Iekhsan" last="Othman">Iekhsan Othman</name><name sortKey="Tee, Ting Yee" sort="Tee, Ting Yee" uniqKey="Tee T" first="Ting Yee" last="Tee">Ting Yee Tee</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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